WebThe CRTI gene was first identified and functionally analyzed by Misawa and coworkers [22] from the soil bacteria Erwinia uredovora, currently renamed as Pantotea ananatis. The pioneering work of Sandman’s group showed that expression of this bacterial CRTI gene in tobacco plants enhanced the production of -carotene. WebMay 1, 2024 · The crtYB (phytoene-β-carotene synthase-PBS) encoding the bifunctional enzyme from Xanthophyllomyces dendrorhous is constitutively expressed in the industrial microalgae C. reinhardtii. This enzyme has both phytoene synthase (PSY) and lycopene cyclization (Lcyb) activities.
UniProt
WebAug 25, 2024 · The color of the strain expressing crtI-c1 was almost similar to the white color of the control strain 50BΔ2549ΔcrtI, indicating an obvious reduction in lycopene production and crtI gene ... WebJun 17, 2024 · When the crtI gene was introduced alone, the rate-limiting enzyme of the upstream MVA pathway was up-regulated 1.39-fold, but the Sc. CEN (B5) strain … how to shake off sleepiness
Cloning and knockout of phytoene desaturase gene in ... - PubMed
WebThe complete crtI gene has been overexpressed in Escherichia coli and R. sphaeroides and shown to catalyze three desaturations of phytoene to give neurosporene. This activity was shown to be ATP dependent, and the cofactor requirement was investigated by using a spectroscopic assay for in vitro carotenogenic activity. WebOct 24, 2024 · Through genetic screening of different varieties, the best combination of carotenase was Taxus x media crtE, Pantoea agglomerans crtB, and B. trispora crtI. When the ypl062w gene was deleted, the cytosolic acetyl … WebMar 23, 2024 · Homologous gene targeting CRTI. We have predicted that the RHTO_04602 locus encode the enzyme CrtI converting phytoene to lycopene for carotenoids biosynthesis in R. toruloides (Zhu et al. 2012).Targeted inactivation of CRTI would result in mutants with visible phenotype differences because of impaired carotenoids formation. So far, reliable … how to shake text in after effects