WebMar 30, 2024 · The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH) assay to assess cell death using a CytoTox 96 non-radioactive cytotoxicity assay kit (Promega). Both the supernatants and cell pellets were collected for immunoblotting analyses. Knockout cell line generation and … WebAbstract. ABSTRACT An injectable ‘biostasis’ drug that could slow organ injury by inducing a state of suspended animation through reversible slowing of metabolic processes would have great value for organ preservation and treatment …
CytoTox-ONE™ Homogeneous Membrane Integrity …
WebCyclosporine A is a potent BSEP inhibitor, however the hepatocyte can adapt through FXR activation of basolateral efflux transporters, and is used as a negative control in the C-DILI Assay. Troglitazone is a potent BSEP inhibitor (sulfate metabolite) and a weak FXR antagonist, and recently the sulfate metabolite has been shown to inhibit OSTα/β5. WebCytoTox-ONE is a trademark of Promega Corporation. Biomek is a registered trademark of Beckman Coulter, Inc. BioWorks is a trademark of Beckman Coulter, Inc. CoStar is a registered trademark of Corning, Inc. MicroMix is a registered trademark of Clintec Nutrition Co. All prices and specifications are subject to change without prior notice. hill walks in lancashire
CytoTox-ONE™ Homogeneous Membrane Integrity Assay - Promega
WebThe CytoTox-ONE™ Assay is a rapid, fluorescent measure of the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the … WebApr 14, 2024 · A CytoTox 96 ® Non-Radioactive Cytotoxicity Assay Kit (Promega) measured the CTL activity. The spleen cells were cultured with 100 U/mL recombinant murine IL-12 for five days as effector cells. The target cells were Sp2/0 mice cells that were transfected with TG_200 mRNA-LNP. WebItem CytoTox 96 Non-Radioactive Cytotoxicity Assay Company Promega Price Supplier Page View Company Product Page Catalog Number G1780 Detection Target Cytotoxicity Quantity 1,000 assays Method Colorimetric Sample Type culture supernatants Assay Time 30 minutes Molecular Weight ~210kDa Promega 2800 Woods Hollow Rd. Madison, … smart business pittsburgh