High-throughput method
WebMar 9, 2024 · High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. We thus developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome these issues. Our improvements … WebSep 21, 2024 · The approach is based on a novel high-throughput method inspired by other known approaches such as thin film PVD (physical vapor deposition) sample generation …
High-throughput method
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WebNov 11, 2024 · Our method offers a simple and high-throughput option for maintaining the quality of plant root microbial community profiling. Introduction The plant root is a key underground organ that... WebApr 14, 2024 · Accurately and rapidly counting the number of maize tassels is critical for maize breeding, management, and monitoring the growth stage of maize plants. With the advent of high-throughput phenotyping platforms and the availability of large-scale datasets, there is a pressing need to automate this task for genotype and phenotype …
WebApr 11, 2010 · To the Editor: High-throughput sequencing is revolutionizing microbial ecology studies. Efforts like the Human Microbiome Projects 1 and the US National Ecological Observatory Network 2 are... WebJun 3, 2024 · We present Microbe-seq, a high-throughput method that yields the genomes of individual microbes from complex microbial communities. We encapsulate individual microbes in droplets with microfluidics and liberate their DNA, which we then amplify, tag with droplet-specific barcodes, and sequence.
WebHigh-throughput sequencing, also known as next-generation sequencing (NGS), is the comprehensive term used to describe technologies that sequence DNA and RNA in a …
WebJul 31, 2024 · In conclusion, our platform provides a high-throughput method to interrogate a wide variety of protein-DNA interactions. The utilities of our DocMF platform can be extended to other applications, such as on-chip identification of off-target sites for a CRISPR-Cas system, single-stranded DNA cleavage sites, or TF binding motifs.
WebMar 8, 2012 · BIPES, a cost-effective high-throughput method for assessing microbial diversity. ISME J 5: 741–749. Article CAS Google Scholar Download references. Acknowledgements. We wish to thank the ... phone drawing with appsWebApr 14, 2024 · Accurately and rapidly counting the number of maize tassels is critical for maize breeding, management, and monitoring the growth stage of maize plants. With the … how do you make powdered sugarWebJun 5, 2024 · To address these shortcomings, we developed Visual Cell Sorting, a flexible and simple high-throughput method that uses commercial hardware to enable the investigation of cells according to visual phenotype. Visual Cell Sorting is an automated platform that directs a digital micromirror device to mark single live cells that express a … how do you make pretendWebDirect infusion of lipid extracts into the ion source of a mass spectrometer is a well-established method for lipid analysis. In most cases, nanofluidic devices are used for sample introduction. ... based on sample infusion from a chromatographic pump can offer a s … Robust and high-throughput lipidomic quantitation of human blood samples ... how do you make prismarine bricksHigh-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology, materials science and chemistry. Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput … See more The key labware or testing vessel of HTS is the microtiter plate, which is a small container, usually disposable and made of plastic, that features a grid of small, open divots called wells. In general, microplates for HTS have either … See more Automation is an essential element in HTS's usefulness. Typically, an integrated robot system consisting of one or more robots transports assay-microplates from station to station for sample and reagent addition, mixing, incubation, and finally readout or … See more Unique distributions of compounds across one or many plates can be employed either to increase the number of assays per plate or to reduce the variance of assay results, or … See more • Chemoproteomics • Compound management • DNA-encoded chemical library See more To prepare for an assay, the researcher fills each well of the plate with some biological entity that they wish to conduct the experiment upon, such as a protein, cells, … See more With the ability of rapid screening of diverse compounds (such as small molecules or siRNAs) to identify active compounds, HTS … See more HTS is a relatively recent innovation, made feasible largely through modern advances in robotics and high-speed computer technology. It still takes a highly specialized and expensive screening lab to run an HTS operation, so in many cases a small- to moderate … See more phone dump july 2018WebA high-throughput LC-MS/MS method for simultaneous determination of direct oral anticoagulants in human plasma J Sep Sci. 2024 Apr 4; e2300084. ... In the present study, … how do you make powdered milkWebThe optimized high-throughput shotgun immunoproteomics approach described herein identifies more than five times as many unique antigens as the previously published method, greatly reduces protocol cost and mass spectrometry time per experiment, minimizes both inter- and intra-experimental variability, and ensures each experiment is fully ... how do you make pozole with pork