Hu-binding site
Web1 Mar 2008 · HU binding to ssDNA, dsDNA and nick in low salt conditions. HU protein, at the concentration indicated in nM on the bottom, was mixed with end-labeled DNA, 40-bp long dsDNA, the same DNA with a ... Web1 Mar 2008 · HU binds single-stranded DNA (ssDNA) in a binding mode that is reminiscent but different from earlier reported specific HU interactions with double-helical DNA …
Hu-binding site
Did you know?
HU is able to bind all forms of nucleic acids, including DNA–RNA hybrids (Balandina et al., 2002). Although HU has no specific binding motif, it is able to bind mRNA of RpoS (Balandina et al., 2001). Another study of the B. subtilis HU protein showed that HU binds an Alu domain of cytoplasmic RNA (Nakamura et al., … See more In 1975, a small, low-molecular-weight DNA-binding protein was isolated from E. coli strain U13 (Rouvière-Yaniv and Gros, 1975). At first, it was referred to as Factor U. Due to its … See more HU protein is among the most abundant and conserved nucleoid-associated proteins in eubacteria (Azam and Ishihama, 1999). The greatest abundance of HU protein has … See more Interaction of HU protein at protein level has not yet been fully explored. As mentioned above, an HU protein is able to react with another HU protein to form a dimer together … See more HU seems to be an important protein for DNA compaction, replication, transcription, recombination, and shape modulation in many bacteria (Broyles and Pettijohn, 1986; Roy et al., 2005; Oberto et al., 2009). In the coccoid … See more Web23 Dec 2008 · Gene expression is potently regulated through the action of RNA-binding proteins (RBPs) and microRNAs (miRNAs). Here, we present evidence of a miRNA regulating an RBP. The RBP HuR can stabilize and modulate the translation of numerous target mRNAs involved in cell proliferation, but little is known about the mechanisms that …
Web8 Jan 2024 · Transcription factor binding sites (TFBSs) are basic components of cis-regulatory elements, which act important roles in precise regulations of gene expressions … Web22 Mar 2011 · HU acts to compact DNA at physiological salt concentrations in vitro by introducing bends ranging from 53° to more than 140° in the DNA as assayed by X-ray …
Web15 Jun 1997 · Site-specific binding of HU is promoted by GalR binding and supercoiled DNA Affinity cleavage mapping by chemically converting HU into a nuclease gives information about the position of the reactive groups in HU in the DNA–protein complex ( Lavoie and Chaconas, 1993; Lavoie et al ., 1996 ). Web27 Feb 2001 · HU, a nonspecific histone-like DNA binding protein, participates in a number of genomic events as an accessory protein and forms multiple complexes …
Web26 Feb 2024 · Starting from a query sequence and predicted I-TASSER models, ATPbind can achieve an average accuracy of 72%, covering 62% of all ATP binding sites while achieving a Matthews correlation coefficient value that is significantly higher than that of other state-of-the-art predictors. Publication types Research Support, N.I.H., Extramural
Web15 Jul 2003 · HU, being a non-specific DNA binding protein, has been more difficult to study. In the IHF case, the hydroxyl radical footprint of >30 bp and the large bend angle observed by FRET in solution agree with the crystal structure ( Yang and Nash, 1989; Lorenz et al ., 2002 ). little earth resident associationWeb20 Dec 2007 · HU binding induces FRET between labeled ssDNA ends. Very long apparent DNA-binding sites and abnormalities in gel mobility of protein–DNA complexes often … little earth prospect backpacksWebCompared to the Mn1-, Mn2- and Mg-doped TiO2 nanocoatings, the Mn3-TiO2 nanocoating significantly upregulated the expression of integrin α5β1 probably through increasing ligand-binding affinity of the integrin rather than integrin binding sites in Fn. little earth rosedale nzWeb19 Mar 2024 · The PWSM has been successfully used in the prediction of transcription factor binding sites and ligand binding sites (Kel et al., 2003; Hu et al., 2016a). Thus, the position weight scoring matrix (PWSM) was used to extract the position conservation information of the basic feature parameters, and the scoring matrix based on amino acid … little earth pune reviewsWeb3 Aug 2009 · Depending on the interacting partner, the binding sites on proteins can be divided into different categories, such as DNA-binding sites, RNA-binding sites, … little earthquakes jennifer weinerWebPapain-like protease (PLpro) is a promising therapeutic target against SARS-CoV-2, but its restricted S1/S2 subsites pose an obstacle in developing active site-directed inhibitors. We have recently identified C270 as a novel covalent allosteric site for SARS-CoV-2 PLpro inhibitors. Here we present a theoreti little earthquakes albumWebHu Proteins Block Binding of CstF64 to Poly(A) Sites and Interact with Two Polyadenylation Factors—To probe the mechanism whereby Hu proteins inhibit … little earth ooty