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Phis1525序列

WebbpHIS1525: 质粒类型: 巨大芽孢杆菌表达载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点,限制性内切酶: 载体大小: 7402 bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: … WebbFor further optimization of our project, more vectors are constructed but not listed here. Some things should be mentioned about plasmid pHIS1525, pSPAsp-hp and pHBintE are that, they are all shuttle vectors for E. coli&B. megaterium. And pHBintE is capable of chromosome integration due to its temperature sensitivity.

pHIS1525载体图谱质粒图谱、序列、价格、抗性、测序引物、大小 …

WebbEkspresi gen dalam sistem pHIS1525/B. megterium -xilosidase ekstraseluler. Tujuan penelitian ini adalah untuk -xilosidase asal IT-08 ke dalam plasmid pHIS1525 dan dipropagasi di . Untuk tahapan penelitian selanjutnya, plasmid rekombinan yang diperoleh di ekspresikan di dalam sistem pHIS1525/ . MATERI DAN METODE Sampel Penelitian 1. Webb2 apr. 2012 · The aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl genewas amplified from pTP510 and cloned into pHIS1525 in E. coli ... simpledateformat wochentag https://group4materials.com

EKSPRESI GEN PENY ANDI E-XILOSID ASE D ALAM SISTEM pHIS1525…

Webb巨大芽孢杆菌系统: phis1525. 巨大宿主菌: yybm1 , wh320 . 乳酸球菌表达系统 pmg36e,nz9000,mg1363 . 乳酸酵母表达系统 pklac1 , gg799 . 白色念珠菌系统 … http://www.xjishu.com/zhuanli/27/202410081742.html WebbPlasmids pHIS1522/pHIS1525 both contain the strong xylA promoter (PxylA)originating from Bacillus megaterium. Transcription from this promoter is xyloseinducible (after xylose addition, the xylose repressor coded by the xylR gene onthe plasmids is released from PxylA and transcription is initiated). raw food diet for cats with urinary crystals

基因的过表达载体构建时,如何设计引物。? - 知乎

Category:pHIS1525巨大芽孢杆菌表达质粒_价格_上海钦诚生物科技有限公司

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Phis1525序列

Bacillus megaterium 発現システム フナコシ

WebbpHIS1525巨大芽孢杆菌表达质粒,下面所有质粒和菌株,均为本公司实验室亲自验证过,可以提供技术支持,载体来源于promega,addgene,clontech,可提供质粒图谱,可提供近万 … WebbFor secretion of fusion proteins, the open reading frames were cloned into the Escherichia coli-Bacillus megaterium shuttle vector pHIS1525. After transformation of the respective plasmids into Bacillus megaterium protoplasts, the recombinant genes were successfully expressed and the proteins were secreted into the growth medium.

Phis1525序列

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Webb16 mars 2016 · 下游引物获得方法我们先 选择cds区最后20(最好25个)个碱基的序列:gaagggcctgactcagactga。然后把这段序列反向互补得到 新的序 … WebbpHIS1525巨大芽孢杆菌表达质粒使用说明: 1、收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒,室温放置1min; 2、从-80℃冰箱中取出相应的感 …

http://www.youbio.cn/product/vt1298 Webb29 sep. 2015 · 提取重组质粒phis1525‐gfp,将其转入已制备好的巨大芽孢杆菌nct‐2原生质体中,转化子能够表达绿色荧光蛋白。 将转化菌株接种到次生盐渍化土壤中,通过监测 …

WebbpHIS1525巨大芽孢杆菌表达质粒人源基因质粒 质粒图谱 质粒序列:详询 质粒菌株产品操作说明书 一、扩增流程 收到产品后,请先根据产品管壁标签来判断产品形式,并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。 1、质粒干粉(常温运输,存于-20度,90天保质期,请务必转化挑单克隆培养,不要直接使用和测序) ①收到质粒干粉后请 … Webb23 jan. 2024 · FISH. 关注. 直接把载体告诉测序公司,如果载体比较常见,公司直接有现成的测序引物提供。. 只需提供质粒或菌液给到公司就行。. 如果载体不常见也可以自己设计 …

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WebbpHIS1525信息由上海博尔森生物科技有限公司为您提供,如您想了解更多关于pHIS1525报价、型号、参数等信息,欢迎来电或留言咨询。除供应pHIS1525外,上海博尔森生物科技有限公司还可为您提供Human KRAS / K-Ras (G12D & Q61H) Protein ... raw food diet for diabetic catshttp://www.nwbiotec.com/index.php?g=&m=article&a=new_nav_list&newid=122 simpledateformat yyyy/mmWebbpHIS1525. PVT13488 2ug. pHIS1525 Search name. pHIS1525,pHIS1525 vector,pHIS1525 plasmid,pHIS1525 map. pHIS1525 Information. Bacterial Resistance(s): Amp Screening: Tet Growth Strain(s): DH5 alpha Growth Condition: LB/37 Centigrade raw food diet for cats with kidney diseaseWebbFor the construction of pHIS1525, fragments from XhoI and BglII digested pMM1525 and pHIS1522 were combined and ligated. The affinity tags of pHIS1525 and pSTREP1525 were combined in one vector using the outlined strategy resulting in pSTREPHIS1525. Next the part of the L. reuteri levansucrase gene, encoding an active and soluble protein in E. raw food diet for dogs nutrition calculatorWebbpHIS1525 Cat.No. VET1404 Host Cell Bacillus megaterium, Escherichia coli Resistance Ampicillin Selection Tetracycline Vector Size 7402 bp Vector Type Expression Vector Q&A Customer Reviews Related Products For research use only. Not intended for any clinical use. Quick Inquiry Service & Products Interested pHIS1525 (VET1404) RESET raw food diet for dachshundsWebbpHIS1525序列pHIS1525图谱/pHIS1525质粒pHIS1525载体 ¥1000 pLVX-EF1伪-AcGFP1-N1序列pLVX-EF1伪-AcGFP1-N1图谱/pLVX-EF1伪-AcGFP1-N1质粒pLVX-EF1伪-AcGFP1-N1载体 ¥1000 pGEX-KG序列pGEX-KG图谱/pGEX-KG质粒pGEX-KG载体 ¥1000 详细信息 询 … simpledateformat yyyy 大文字Webb22 dec. 2014 · without changing the N-terminus of the protein of interest. pHIS1525 contains a sequence encoding the B. subtilis extracellular esterase (LipA) and allows … simpledateformat类的parse