Phospho eif2 alpha antibody

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August undefined, 2024

WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of … WebPhospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51. Formulation： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source： Polyclonal, Rabbit,IgG Dilution： IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000.

Human Phospho-eIF2 alpha (S52) Antibody MAB39971: …

WebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … WebAnti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51. Tested applications Suitable for: WB, IHC-P, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF or IP Species … bitbucket verify committer

The structural basis of translational control by eIF2 ... - Nature

WebProduct Name eIF2 alpha (Phospho-Ser51) Rabbit mAb; Host Species Rabbit; Clonality Monoclonal; Isotype Rabbit IgG; Purification Affinity-chromatography; Applications WB … WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography. Validations None provided Applications Western Blotting (WB) Reactivity Chlorocebus sabaeus (Green monkey) WebPhospho-EIF2S1 (Ser51) antibody for WB, ELISA and reacts with Human, mouse, rat. ... eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51 ... EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis ... darwin cyclone shelters

Phospho-eIF2α (Ser51) Antibody Cell Signaling Technology

Anti-eIF2 alpha (phospho Ser48) antibody (GTX50342)

WebGuarantees. Rabbit polyclonal antibody to eIF2 alpha (phospho Ser51). This antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser51. Synthetic … WebYour search returned 47 eIF2 alpha, phospho Antibodies across 9 suppliers. Showing 9 of 9 suppliers (47 products total) > >> Select All. Select up to 5 products from below to compare or request more information. Request Info for all … bitbucket version controlWebPhosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important … bitbucket verify commit signature

"WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … " - Phospho eif2 alpha antibody

Phospho eif2 alpha antibody

Anti-eIF2 alpha, phospho Antibody Products Biocompare

WebPhospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize … WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of …

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WebMay 11, 2024 · Human Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm …

WebAntibodies are purified by protein A and peptide affinity chromatography. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism of downregulating protein … http://immunoway.com/Home/22/YP0093

WebSubunit Structure: Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. … WebMay 13, 2024 · For eIF2 phosphorylation, purified PKR and eIF2 (0.08 µg PKR per 10 µg of eIF2) were mixed at room temperature in eIF2 storage buffer supplemented with 0.5 mM ATP, 10 mM MgCl 2 and 5 mM NaF, and ...

WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ...

WebJul 16, 2024 · eIF2α Antibody (D-3) is an IgG 1 κ mouse monoclonal eIF2 alpha antibody (also designated EIF2A antibody, eIF2 alpha antibody, or EIF2S1 antibody) suitable for the … bitbucket view file historyWebEIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa. Protocols Publications All (7) KD/KO (1) WB (7) IP (2) bitbucket view deleted branchesWebNov 26, 2024 · Phospho-eIF2α has increased affinity to the eIF2β subunit and prevents the regeneration of guanosine triphosphate (GTP) in the ternary complex eIF2-GTP-Met tRNAi, thus halting the initiation of ... bitbucket visual studio invalid credentialsWebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ... bitbucket vocalink.co.ukWebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. darwin cydia repository managerWebEIF2 alpha antibody; EIF2 antibody; EIF2A antibody; EIF2S1 antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody; ... All lanes : Anti-EIF2S1 (phospho S51) antibody (ab131505) at 1/500 dilution Lane 1 : K562 cell extract Lane 2 : K562 cell extract treated with IFN alpha bitbucket vs codecommitWebPhospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb detects endogenous eIF2α only when phosphorylated at Ser51. The antibody does not recognize elF2α phosphorylated at other … darwin cyclone tracy museum